Polytechnic University of Valencia Congress, 21st International Drying Symposium

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Study and optimization of freeze-drying cycles of a model probiotic strain
Pierre Verlhac, Séverine Vessot-Crastes, Ghania Degobert, Claudia Cogné, Julien Andrieu, Laurent Beney, Patrick Gervais

Last modified: 26-12-2018


This work is based on the experimental study of the freeze-drying process to understand the impact of numerous factors on the survival rates of a model probiotic strain of Lactobacillus casei type. With the aim to find out if cell density in the matrix and survival rates are linked, we have studied the location of the cells after freeze drying inside a porous  matrix composed of a lactose basis with a polymer, the polyvinylpyrrolidone (PVP) in various amounts. The best survival rate were obtained at slow freezing rate for a formulation containing 5% (m/V) of lactose and 5% (m/V) of PVP.

Keywords: Freeze-Drying; Freezing; Probiotics; L. Casei ATCC 393


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