Polytechnic University of Valencia Congress, 21st International Drying Symposium

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Long-term maintenance of dried acellular matrices
Alessandro Zambon, Giovanni Giobbe, Massimo Vetralla, Filippo Michelino, Luca Urbani, Maria Pantano, Nicola Pugno, Paolo De Coppi, Nicola Elvassore, Sara Spilimbergo

Last modified: 03-01-2019


Dried and sterile acellular esophageal matrix was obtained within a new drying process based on the use of supercritical carbon dioxide (SC-CO2). Experiments were performed coupling a conventional detergent enzymatic treatment with two different drying methods: (i) SC-CO2 drying alone; (ii) dehydration in ethanol and a subsequent SC-CO2 drying. Long term preservation was achieved for several months after drying, demonstrating the maintenance of extracellular matrix (ECM) structure, mechanical properties and biocompatibility within cell repopulation studies in vitro. Overall, the results highlighted the potential of this novel technology to obtain a dry and sterile acellular matrix that can be easily stored for oesophageal regeneration in patients with emergency need.

Keywords: decellularized tissue; supercritical drying; carbon dioxide; tissue engineering; long term storage

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